犬抗鑰孔戚血藍素IgG檢測試劑盒

Canine Anti-Keyhole limpet hemocyanin (KLH) IgG ELISA Kit

cat#700-195-KLG

INTENDED USE

The Canine Anti-KLH IgG ELISA in an indirect ELISA for the detection of and quantification of KLH (keyhole limpet hemocyanin)-specific IgG in canine serum or plasma. This immunoassay is suitable for:

1）Determining immune status relative to non-immune controls;

2）Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;

3）Qualifying and/or standardizing vaccine batches and protocols.

4）Measuring natural anti-KLH antibodies of innate immunity.

GENERAL INFORMATION

Keyhole Limpet Hemocyanin (KLH), an oxygen-transporting protein of the marine gastropod Megathura crenulata, is recognized as a potent immunoactivator, and therefore is widely used in research and clinical studies. Present applications of KLH include: (a) use as a highly immunogenic antigen for assessment of immune competence of an organism, and (b) frequent use as acarrier of low molecular mass peptides and haptens, such as oligosaccharides, gangliosides or (glyco)peptides, designed to facilitate antibody production. In these cases, antibodies made to small peptides/haptens are generally raised by coupling to a large carrier protein like KLH. Antibodies are produced to both KLH and the peptide/hapten; because anti-KLH may give non-specific signals in various immunoassays, removal by solid phase immunoaffinity chromatography is common. The ELISA is useful for determining levels of anti-KLH in sera and for monitoring anti-KLH removal from purified samples after antibody production. The ADI anti-KLH ELISA is designed with high sensitivity for discriminating lower level antibodies, which may include natural anti-KLH antibodies of innate immunity, with specially formulated diluents to minimize interfering background signals.

PRINCIPLE OF THE TEST

The Canine Anti-KLH IgG ELISA kit is based on the binding of Canine anti-KLH in samples to KLH antigen immobilized on the microwells, and anti- KLH IgG antibody is detected by anti-Canine IgG-specific antibody conjugated to HRP conjugate. After a washing step, chromogenic substrate (TMB) is added and color (blue), which is directly proportional to the amount of antibody present in the sample. Stop Solution is added (converts blue to yellow color), and A450nm is then measured using an ELISA reader. The activity of antibody in samples is calculated relative to supplied calibrators.

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